Increased abundance and transferability of resistance genes after field application of manure from sulfadiazine-treated pigs.
نویسندگان
چکیده
Spreading manure containing antibiotics in agriculture is assumed to stimulate the dissemination of antibiotic resistance in soil bacterial populations. Plant roots influencing the soil environment and its microflora by exudation of growth substrates might considerably increase this effect. In this study, the effects of manure from pigs treated with sulfadiazine (SDZ), here called SDZ manure, on the abundance and transferability of sulfonamide resistance genes sul1 and sul2 in the rhizosphere of maize and grass were compared to the effects in bulk soil in a field experiment. In plots that repeatedly received SDZ manure, a significantly higher abundance of both sul genes was detected compared to that in plots where manure from untreated pigs was applied. Significantly lower abundances of sul genes relative to bacterial ribosomal genes were encountered in the rhizosphere than in bulk soil. However, in contrast to results for bulk soil, the sul gene abundance in the SDZ manure-treated rhizosphere constantly deviated from control treatments over a period of 6 weeks after manuring, suggesting ongoing antibiotic selection over this period. Transferability of sulfonamide resistance was analyzed by capturing resistance plasmids from soil communities into Escherichia coli. Increased rates of plasmid capture were observed in samples from SDZ manure-treated bulk soil and the rhizosphere of maize and grass. More than 97% of the captured plasmids belonged to the LowGC type (having low G+C content), giving further evidence for their important contribution to the environmental spread of antibiotic resistance. In conclusion, differences between bulk soil and rhizosphere need to be considered when assessing the risks associated with the spreading of antibiotic resistance.
منابع مشابه
Effects of Veterinary Medicines Introduced via Manure into Soil on Microbial Communities
Multiple antibiotic resistant bacterial pathogens pose a major threat for the successful therapeutic use of antibiotics in human and veterinary medicine. Veterinary antibiotics used in animal husbandry are assumed to contribute to an increased antibiotic resistance among bacteria in manure. Bacteria carrying transferable antibiotic resistances, antibiotics, metabolites and nutrients are inevita...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 79 5 شماره
صفحات -
تاریخ انتشار 2013